During the screening for new antibiotics, a new antibiotic was isolated in crystalline form from the cultured broth of a Streptomyces, isolated from a soil sample collected in Okinawa. The structural studies revealed that this antibiotic was deoxynybomycin1}, which had been already derived chemically from nybomycin2) by Rinehart et al. In this communication, characteristics of the strain, production, isolation, properties of this antibiotic, and the structural determination are reported.