<jats:title>Abstract</jats:title><jats:p>Twenty‐nine disorazoles A–H (<jats:bold>1–29</jats:bold>) were isolated by solvent partitions and chromatographic separations from <jats:italic>Sorangium cellulosum</jats:italic>, strain So ce12, the producer of the sorangicin antibiotics. The disorazoles proved to be highly cytotoxic and active against fungi. The structures of the main component disorazole A<jats:sub>1</jats:sub> (<jats:bold>1</jats:bold>) and 28 variants were elucidated by 2D‐NMR and mass spectroscopy. The disorazoles are macrocyclic dilactones of two 2‐pentadecyloxazol‐4‐carboxylic acids, which are modified in their carbon chain by variation of the position and configuration of double bonds and oxygen substituents like epoxide, hydroxyl, or methyl ether groups. In addition to these, three disorazoles are ring‐enlarged by lactonization to a more distant hydroxyl group. By feeding of <jats:sup>13</jats:sup>C‐enriched precursors the biosynthetic origin of the carbons in disorazole A (<jats:bold>1</jats:bold>) was investigated. C‐2 of the oxazole and the attached pentadecyl chain arise from acetate. The geminal methyl groups and the methoxyl group are derived from the methyl group of methionine. <jats:chem-struct-wrap><jats:chem-struct><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/gif" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/must001.gif"><jats:alt-text>magnified image</jats:alt-text></jats:graphic></jats:chem-struct></jats:chem-struct-wrap>