<jats:title>Abstract</jats:title><jats:p>A new alkaloid, oximoaspergillimide (<jats:bold>1</jats:bold>), and the known neohydroxyaspergillic (<jats:bold>2</jats:bold>) and neoaspergillic (<jats:bold>3</jats:bold>) acids, have been isolated from the extract of a cultured marine‐derived fungus (strain CF07002) identified as a member of the genus <jats:italic>Aspergillus</jats:italic>. The structure of <jats:bold>1</jats:bold> was determined by interpretation of NMR spectroscopic data, and confirmed by synthesis. The antimicrobial and cytotoxic activities of compounds <jats:bold>1</jats:bold>–<jats:bold>3</jats:bold> were evaluated.