A new alkaloid, oximoaspergillimide (1), and the known neohydroxyaspergillic (2) and neoaspergillic (3) acids, have been isolated from the extract of a cultured marine-derived fungus (strain CF07002) identified as a member of the genus Aspergillus. The structure of 1 was determined by interpretation of NMR spectroscopic data, and confirmed by synthesis. The antimicrobial and cytotoxic activities of compounds 1-3 were evaluated.