<jats:p>Extensive chromatographic purification of the alkaloid fraction of Aconitum vulparia Rchb. led to the isolation of a new norditerpene alkaloid, vulparine (1), besides the known compounds septentriodine (2), finetiadine (3), anthranoyllycoctonine (4), N-methyl-N-deethyllycoctonine (5) and delectinine (6). The structure of the new compound was determined by means of HRMS, 1D and 2D NMR spectroscopy. Detailed NMR studies, including <jats:sup>1</jats:sup>H-<jats:sup>1</jats:sup>H COSY, NOESY, HSQC and HMBC experiments, resulted in complete and unambiguous <jats:sup>1</jats:sup>H chemical shift assignments for 2 and 6, and revision of some <jats:sup>13</jats:sup>C NMR data. Compounds 1 - 4 were evaluated for their cytotoxic activities, and 1, 3 and 4 were found to exhibit marginal cell growth inhibitory activity against breast adenocarcinoma (MCF-7) and cervix adenocarcinoma (HeLa) cells.