Two new C-19-diterpenoid alkaloids, named villosudine A (1) and villosudine B (2), along with seven known diterpenoid alkaloids, were isolated from the root of Aconitum franchetii var. villosulum. Their structures were elucidated by extensive spectroscopic analyses including 1D, 2D NMR, and HR-ESI-MS. Compounds (1-7) were evaluated for their cytotoxicity against the MCF-7 and HepG2 human cancer cell lines.