Eight new alkaloids (1e8), were isolated from the solid culture of Hericium erinaceus. The structures of new metabolites were established by spectroscopic methods. The absolute configuration of 1 was determined by electronic circular dichroism (ECD) calculation. Compounds 5e8 showed inhibitory activities against both protein tyrosine phosphatase-1B (PTP1B) and a-glucosidase. In the cytotoxicity assay, compounds 1e4 showed moderate cytotoxicity against K562 cells with IC50 values in the range of 11.4e18.2 mM, and also weak cytotoxicity against doxorubicin resistant K562 cells.