Eight new alkaloids (1-8), were isolated from the solid culture of Hericium erinaceus. The structures of new metabolites were established by spectroscopic methods. The absolute configuration of 1 was determined by electronic circular dichroism (ECD) calculation. Compounds 5-8 showed inhibitory activities against both protein tyrosine phosphatase-1B (PTP1B) and alpha-glucosidase. In the cytotoxicity assay, compounds 1-4 showed moderate cytotoxicity against K562 cells with IC50 values in the range of 11.4-18.2 mu M, and also weak cytotoxicity against doxorubicin resistant K562 cells. (C) 2015 Elsevier Ltd. All rights reserved.