Arundo donax L. is a perennial reed that grows very common in the Nile region, Oases of the Egyptian Libyan desert, The Mediterranean coastal region, The Isthmix desert and Sinai. The alkaloids are more concentrated in the underground organs than the aerial parts, reaching up to 0.05% as total alkaloids. Five spots were identified by TLC using different solvent systems (detected by UV light and van Urk's reagent); among which three alkaloids were isolated and identified by their melting point (m.p.), mixed melting point (m.m.p.), chromatographic behaviour and spectral data: Gramine (N,N-dimethyl-1H-indole-3-methanamine) with m.p. 138—139°C; N,N-dimethyl tryptamine with m.p. 445—470°C (its picrate m.p. 169—170°C); and hufotenine (%hydroxy-N-dimethyltryptamine) with m.p. 146—147°C.