The genus Kitasatospora comprises a group of filamentous and aerobic Gram-positive bacteria and is phenotypically closely related to Stereptomyces strains. Characteristics of the genus Kitasatosporia are the presence of meso-diaminopimelic acid, LL-diaminopimelic acid, and galactose in the cell wall.1,2) During the course of our search for antifungal metabolites from microbes, we found that methanolic extracts of the culture broth of a new species of the genus Kitasatosporia isolated from a soil sample inhibited the growth of plant pathogens, such as Rhizoctonia solani, Botrytis cinerea, and Phythium ultimum. Classification and characteristics of the species have been reported.3) In this paper, we report the isolation, structure determination, and biological activities of a new bafilomycin derivative 2 designated bafilomycin C1-amide as well as the known bafilomycins, 1, 3, 4, and 5 from the fermentation broth.