The content of glucosinolates and amines in green parts of Reseda media has been investigated. Benzylglucosinolate, 2-phenethylgiucosinolate, and m-hydroxybenzylglucosinolate occur in appreciable amounts accompanied by minor amounts of other glucosinolates, benzylamine and m-hydroxybenzylamine. Isolation and identificationofthesecompoundswasmadeusingion-exchangechromatography, highvoltageelectrophoresis,GC,MS, and 13C-NMR spectroscopy. The glucosinolates were transformed into corresponding nitriles and isothiocyanates by thioglucoside glucohydrolase-catalysed hydrolysis and to the corresponding carboxylic acids by acid-catalysed hydrolysis. Thecontent ofglucosinolatesand amines in leaves and inflorescencesofR. media has been determined by UVspectroscopy and GC.