The neurological disorder, Dallisgrass poisoning or paspalum staggers, occurs in cattle that graze Paspalum dilatatum infected with the fungus Claviceps paspali and occurs sporadically in the southern portions of the US. Prompted by the availability of field-produced sclerotia and the observation that paxilline (I), a tremorgenic metabolite of Penicillium paxilli (Cole et al., 1974; Springer et al., 1975), was closely related to previously reported metabolites [paspaline (II), paspalicine (III), and paspalinine (IV)] of Claviceps paspali (Fehr and Acklin, 1966), we analyzed sclerotia of this fungus for chemically related tremorgenic metabolites. Three tremorgenic metabolites have been isolated from C. paspali sclerotia collected from Paspalurn dilatatum. The identification of these metabolites has been accomplished by spectroscopic methods. One of the metabolites is identical with paspalinine, a previously reported metabolite of C. paspali. The remaining two metabolites differ from paspalinine in that they contain an additional isoprene and hydroxyisoprene unit attached to carbon 5 of the six-membered indole ring, 3-methyl-2-butenylpaspalinine and 3-hydroxy-3-methyl-1-butenylpaspalinine, respectively.