<jats:p> A purple pigment, named cochliodinol, C<jats:sub>32</jats:sub>H<jats:sub>32</jats:sub>N<jats:sub>2</jats:sub>O<jats:sub>4</jats:sub>, has been isolated from three isolates of Chaetomium cochliodes and from two isolates of Chaetomium globosum. The pigment is produced intracellularly on a wide range of media in quantities up to 0.45 mg/ml. The rate of production for a given isolate is proportional to its growth but such proportionality is not observed when one isolate is compared to another. A second, fluorescent pigment, C<jats:sub>32</jats:sub> H<jats:sub>28</jats:sub>N<jats:sub>2</jats:sub>O<jats:sub>4</jats:sub>, is produced by those isolates that produce cochliodinol but in 1/500 of the yield.