A new alkaloid tamynine has been isolated from Murraya paniculata. Its structure has been assigned as 1 on the basis of chemical and spectral studies. Murraya paniculata (Rutaceae), locally called "Orange Jasmine" or "China pox" is commonly found in Karachi, Pakistan as an ornamental plant. It is very popular for its green foliage and large clusters of fragrant flowers. It is propagated by seeds, cutting or layering. Murraya paniculata belongs to family Rutaceae which is spread out in the tropical and subtropical regions of South East Asia, Mediterranian countries, North America, Australia and South Africa. The leaves are useful for the treatment of diarrhoea, dysentry and posses antibiotic activity against the Micrococcus pyogen var. aureus and E. coli. Powdered leaves are applied to cuts. Twigs are used for dental cleaning purposes. Earlier investigations on various parts of this plant led to the isolation of a few alkaloids, i.e. paniculidine, noracronycine, skimmianine and yuehhuchene. In view of our continued interests in the chemical constituents of this plant, a reinvestigation in the leaves of this plant was undertaken. As a result of preliminary work on the leaves of Murraya paniculata a new crystalline alkaloid (Tamynine) has been isolated of which structure (1) has been assigned on the basis of extensive nmr studies. The ¹H nmr assignments were made by means of ¹H-¹H, NOESY, COSY, J-resolved 2D homodecoupling and n.o.e experiments. The ¹³C multiplicities were established by carrying out DEPT experiments.