Cycloclavine and other indolealkaloids were found in a fungus isolated from soil near the laboratory, which was identified as a strain of Aspergillus japonicus SAITO based on morphological and physiological properties (consistent with A. japonicus SAITO IFO 4060). The fungus was cultivated in a medium containing 3% mannitol, 1% glucose, 1% succinic acid, 0.1% KH₂PO₄, 0.03% MgSO₄·7H₂O (pH 5.6) at 27°C for 7 days on a rotary shaker. The mycelia and culture filtrate were processed via acid extraction, alkalization, ethylacetate extraction, and silica gel column chromatography to isolate two alkaloids (AJ-1 and AJ-2). AJ-1 was identified as cycloclavine by melting point, optical rotation, UV, IR, MS, NMR, elemental analysis, color reactions, and mixed melting point with an authentic sample. AJ-2 was identified as festuclavine by similar analyses including melting point, optical rotation, MS, elemental analysis, and mixed melting point.