In 1969, Stauffacher et al.1] isolated a new clavine-type ergot alkaloid together with some festuclavine from the seeds of Kenyan Ipomoea hildebrandtii Vatke. The compound was named cycloclavine according to its pentacyclic structure as shown here. Now, we have found cycloclavine and some other indolealkaloids in a fungus which was isolated from soil near our laboratory. On the basis of morphological and physiological properties, this fungus was assigned to a strain of Aspergillus japonicus Saito.2>3) Moreover, the said properties of this fungus were in good agreement with those of A.japonicus Saito IFO 4060. For the production of alkaloids, the organism was cultivated on a rotary shaker (220 rpm) at 27°C for 7 days in 500-ml Erlenmeyer flasks, each containing 100ml of medium which consisted of 3% mannitol, 1% glucose, 1% succinic acid, 0.1% KH2PO4, 0.03% MgSO4·7H2O, and NH4OH to adjust the pH to 5.6. The mycelia were separated by filtration and extracted with 0.1 N H2SO4. The acid extract was combined with the culture filtrate (ca. 45 liters) and brought to pH 10 by the addition of NH4OH. The mixture was extracted repeatedly with ethyl acetate. The extract was concentrated to dryness under reduced pressure, and the residue was dissolved in chloroform. The chloroform solution was shaken with 1% phosphoric acid (pH ca. 2) to transfer alkaloids into the acidic phase, which was transferred back into chloroform upon pH adjustment to 10 with NH4OH. The chloroform solution obtained was concentrated to dryness and the residue was adsorbed on a silica gel column (Wakogel 30 g, 02.5 x 10cm) and eluted with a mixture of chloroform-methanol (95 : 5 v/v). Two alkaloids named AJ-1 and AJ-2 were eluted separately from each other.