<jats:p> Browniine, dehydrobrowniine, lycoctonine, hetisine, and a dehydrohetisine were isolated from Delphiniumcardinale Hook. Delatine, an alkaloid previously isolated from D. elatum L., was found to be hetisine. These are the first examples of the occurrence of the Aconitum alkaloid hetisine in Delphinium spp.