<jats:title>Abstract</jats:title><jats:p>Seven new steroidal alkaloids, 2‐hydroxysalignarine‐E (=(2′<jats:italic>E</jats:italic>,20<jats:italic>S</jats:italic>)‐20‐(dimethylamino)‐2<jats:italic>β</jats:italic>‐hydroxy‐3<jats:italic>β</jats:italic>‐(tigloylamino)pregn‐4‐ene; <jats:bold>1</jats:bold>), 5,6‐dihydrosarconidine (=(20<jats:italic>S</jats:italic>)‐20‐(dimethylamino)‐3<jats:italic>β</jats:italic>‐(methylamino)‐5<jats:italic>α</jats:italic>‐pregn‐16‐ene; <jats:bold>2</jats:bold>), salignamine (=(20<jats:italic>S</jats:italic>)‐20‐(methylamino)‐3<jats:italic>β</jats:italic>‐methoxypregna‐5,16‐diene; <jats:bold>3</jats:bold>), 2‐hydroxysalignamine (=(20<jats:italic>S</jats:italic>)‐20‐(dimethylamino)‐2<jats:italic>β</jats:italic>‐hydroxy‐3<jats:italic>β</jats:italic>‐methoxypregna‐5,16‐diene; <jats:bold>4</jats:bold>), salignarine‐F (=(2′<jats:italic>E</jats:italic>, 20<jats:italic>S</jats:italic>)‐20‐(dimethylamino)‐4<jats:italic>β</jats:italic>‐hydroxy‐3<jats:italic>β</jats:italic>‐(tigloylamino)pregn‐5‐ene; <jats:bold>5</jats:bold>), salonine‐C (=(2′<jats:italic>E</jats:italic>,20<jats:italic>S</jats:italic>)‐20‐(dimethylamino)‐3<jats:italic>β</jats:italic>‐(tigloylamino)pregna‐4,14‐diene; <jats:bold>6</jats:bold>), and <jats:italic>N</jats:italic>‐[formyl(methyl)amino]salonine‐B (=(20<jats:italic>S</jats:italic>)‐20‐[formyl(methyl)amino]‐3<jats:italic>β</jats:italic>‐methoxypregna‐5,16‐diene; <jats:bold>7</jats:bold>) have been isolated from the MeOH extract of <jats:italic>Sarcococca saligna</jats:italic>, along with the six known alkaloids dictyophlebine (<jats:bold>8</jats:bold>), epipachysamine‐D (<jats:bold>9</jats:bold>), saracosine (<jats:bold>10</jats:bold>), iso‐<jats:italic>N</jats:italic>‐formylchonemorphine (<jats:bold>11</jats:bold>), sarcodinine (<jats:bold>12</jats:bold>), and alkaloid‐C (<jats:bold>13</jats:bold>). The structures of <jats:bold>1</jats:bold>–<jats:bold>7</jats:bold> were deduced from spectral data. Compounds <jats:bold>1</jats:bold>–<jats:bold>13</jats:bold> demonstrated significant activity against acetyl‐ and butyrylcholinesterase.