A novel indole alkaloid, nauclefolinine (1) and five known triterpenic compounds, rotundic acid (2), α-L-rhamnoquinovic acid (3), 3-O-β-D-glucopyranosyl-β-sitosterol (4), squalene (5) and sitosterol-3-O-6'-stearoyl-β-D-glucopyranoside (6) have been isolated from the roots of Nauclea latifolia.