Entomopathogenic fungi seem to be interesting as a source of biologically active compounds such as insecticides1) and immunosuppressants2,3), because all insect orders are susceptible to fungal diseases. With the goal of finding novel bioactive compounds from fungi, we investigated metabolite diversity using an HPLC screening program. During the screening process, we isolated two novel metabolites named pyridovericin (1) and pyridomacrolidin (2) (Fig. 1) from the mycelial cake of the entomopathogenic fungus, Beauveria bassiana EPF-54). In this paper, we describe the physico-chemical properties and structural determination of 1 and 2.