Many entomopathogenic fungi are presumed to cause host death through the inhibition of enzymes and interference with the regulatory system in the host by production of toxic fungal metabolites and pathogenic enzymes1~3). The entomopathogenic fungi can be classified into five genera: Oomycota, Chytridiomycota, Zygomycota, Ascomycota, and Deuteromycota, and their hosts are various4~6). Among these fungi, Deuteromycota seems to be preferred as a source of screening for new bioactive compounds from fungal metabolites as fungi can be cultured on artificial media. So far, many bioactive compounds with insecticidal, antifungal and immunosuppressive activity have been isolated successfully from entomopathogenic fungal metabolites7~12). In the course of our HPLC screening program, we systematically investigated the diversity of metabolites in Deuteromycota, Beauveria bassiana, Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces fumosoroseus and Verticillium lecanii, which have been isolated from the corpses of insects. Comparative study of the HPLC elution pattern among fungal metabolites found that a strain, Beauveria bassiana EPF-5, produced two novel metabolites, designated pyridovericin (1) and pyridomacrolidin (2). In this communication, we preliminarily report the production, isolation, structure and biological activities of 1 and 2.