The pyrrolizidine alkaloid (PA) fraction of two Senecio species (Senecio inaequidens and Senecio cordatus) was extracted by off-line supercritical fluid extraction (sfe) and analyzed by capillary gas chromatography. Sfe was carried out with a home-assembled apparatus, using MeOH/CO₂ as extraction medium, at 55° and 15 MPa. Compared to the classical MeOH Soxhlet extraction, this technique requires a smaller sample amount and provides a quicker extraction, a simplified PA fraction clean-up, and a higher recovery.