Thalictrum glandulosissimum (Finet et Gagnep.) W. T. Wang et S. H. Wang (Ranunculaceae) is an ancient perennial herb of China with a history of use as a medicinal in the therapy of acute infections. In a continuation of an earlier study in which the isolation of quaternary alkaloids from an extract of the rhizomes and roots of this plant was described, this paper reports the isolation and identification of seven alkaloids from the non-quaternary fractions of that extract. Extensive gradient column chromatography (CHCl3-MeOH mixtures, silica gel) and preparative TLC (C6H6-Me2CO-NH4OH mixtures, silica gel) of the non-quaternary alkaloid fractions afforded the protopine alkaloids cryptopine (62 mg) and protopine (35 mg); the bishenzylisoquinoline alkaloids (+)-hernandezine (38 mg) and (+)-thalidezine (32 mg); the protoberberine alkaloids 8-oxocoptisine (22 mg) and berberrubine (8 mg); and the possibly artefactual ketolactam (±)-puntarenine (18 mg). The alkaloids were identified by direct comparison (UV, IR, 1H-NMR, EI-MS, m.p., optical activity) with authentic samples and/or published data. Numerous bioactivities have been associated with cryptopine, protopine, (+)-hernandezine, and (+)-thalidezine, including antimicrobial, hypotensive, hypothermic, anti-inflammatory, oxytocic, skeletal muscle relaxant, anticoagulant, and cardiovascular effects. Although these four alkaloids were recently reported as constituents of T. glandulosissimum (in addition to isothalidezine, O-methylthalibrine, and izmirine) and have been found in other Thalictrum sp., this is the first reported isolation of 8-oxocoptisine and puntarenine from the genus Thalictrum.