The dried and ground rhizomes and roots of Thalictrum glandulosissimum were defatted with petroleum ether, extracted with ethanol, and the ethanol extract fractionated into non-phenolic, phenolic non-quaternary, and quaternary alkaloid fractions. Chromatography of the quaternary alkaloid chloride fraction afforded seven quaternary protoberberine alkaloids (berberine, palmatine, coptisine, thalifendine, groenlandicine, columbamine, jatrorrhizine) and one quaternary aporphine alkaloid (magnoflorine), identified by comparison with authentic samples (UV, IR, m.p., [α]D) and published data. This is the first reported isolation of alkaloids from T. glandulosissimum, and the first isolation of coptisine and groenlandicine from the genus Thalictrum; groenlandicine is only the second reported from nature. For Genista involucrata (endemic to South and Central Anatolia) and Genista albida, the total alkaloid contents of aerial parts were 0.13% and 0.15%, respectively. Alkaloids were isolated by preparative TLC and identified: from G. involucrata, (-)-sparteine, (+)-17-oxosparteine, (+)-lupanine, 13-epimethoxylupanine, anagyrine, N-methylcytisine, cytisine, (+)-calycotomine; from G. albida, (+)-17-oxosparteine, (+)-lupanine, 13-epimethoxylupanine, anagyrine, N-methylcytisine, N-acetylcytisine. 13-Epimethoxylupanine and N-acetylcytisine were obtained for the first time from the genus Genista.