There is practically no information in the literature on the lipids of tomato stems but only reports of the fatty acid composition of tomato leaves. We studied the stems of a tomato (Lycopersicum esculentum) growing in Uzbekistan. The air-dry comminuted tomato stems were extracted with a chloroform-methanol mixture, with the yield of extractive substances being 8.2% of the stem weight, and the amounts of unsaponifiable substances, carotenoids, and chlorophylls in the extract being 19.0%, 1.2%, and 1.8% of the lipid weight, respectively. Neutral and polar lipid classes were identified: neutral lipids included hydrocarbons, carotenoids, esters of sterols and triterpenols with fatty acids, aliphatic alcohols, tocopherols, free fatty acids, triterpenols, sterols, and chlorophylls and their derivatives; phospholipids included phosphatidylethanolamine, phosphatidylcholine (predominant), phosphatidylinositol, lysophosphatidylcholine, and phosphatidic acid; glycolipids included steryl glycosides and their esters with fatty acids, sulfoquinovosyldiacylglycerols, and mono- and digalactosyldiacylglycerols (monogalactosyldiacylglycerols predominant). Total fatty acids were isolated by hot saponification with 10% methanolic KOH and analyzed as methyl esters by GLC, with the composition (% on acid weight): 10:0 (tr.); 12:0 (0.5); 14:0 (0.9); 15:0 (2.8); 16:0 (27.8); 16:1 (4.8); X (0.9); 17:0 (1.4); 18:0 (4.4); 18:1 (2.5); 18:2 (16.0); 18:3 (25.4); 22:0 (12.6). The high content of 18:3 acid is characteristic of leaf lipids of most higher plants, and an acid with more than 18 carbon atoms was also detected. Results show the tomato stem extract contains biologically active components analogous to those in previously studied materials with practical use.