Six new steroidal alkaloids were isolated from roots of a hybrid of Lycopersicon esculentum x L. hirsutum and their structures elucidated as 5α-tomatidan-3-one, (23R)-23-acetoxytomatidine, (23S)-23- acetoxysoladulcidine, (23S,25S)-23-acetoxy-5α,22αN-spirosolan-3β-o1; 22,26-epimino- 16β,23-epoxy-23α-ethoxy-5α,25αH-cholest-22(N)-ene-3β,20α-diol and 22,26-epimino-16β, 23-epoxy-5α,22βH,25αH-cholestane-3β,23α-diol, respectively. © 1993.