6-Aminopenicillanic acid II. Formation of 6-aminopenicillanic acid by Emericellopsis minima (Stolk) and related fungi

Abstract

<jats:p> In submerged culture, <jats:italic>Emericellopsis minima</jats:italic> (Stolk) I. M. I. 69015 produced a substance resembling 6-aminopenicillanic acid in its behaviour on paper chromatograms and in its properties after reaction with phenylacetyl chloride. The substance was destroyed by peni­cillinase but was acid-stable. The culture also produced cephalosporin <jats:italic>N</jats:italic> and two unidentified antibiotics which have been named emericellopsin <jats:italic>A</jats:italic> and <jats:italic>B</jats:italic> . When other fungi of the genera <jats:italic>Emericellopsis</jats:italic> and <jats:italic>Gephalosporium</jats:italic> were examined in a similar manner, <jats:italic>C</jats:italic> , <jats:italic>salmosynnematum</jats:italic> and <jats:italic>Cephalosporium</jats:italic> I. M. I. 49137 were also found to produce small amounts of a substance resembling 6-aminopenicillanic acid. Examination of <jats:italic>Emericellopsis minima</jats:italic> (Stolk) also showed the presence of penicillin amidase activity in this culture.