Using HPLC-UV(DAD)-MS the MeOH/H2O/HOAc extract of the roots of Aphelandra fuscopunctata (Acanthaceae) was analyzed. The known alkaloid (+)-aphelandrine and two new macrocyclic spermine alkaloids of the (+)-aphelandrine type were isolated, namely (+)-N(6)-hydroxy-aphelandrine and (+)-N(6)-acetoxy-aphelandrine. Their constitutions and absolute configurations were established. (+)-N(6)-Hydroxy-aphelandrine was obtained from (+)-aphelandrine and (+)-N(6)-acetoxy-aphelandrine. (C) 1999 Elsevier Science Ltd. All rights reserved.