Thalictrum orientale (Ranunculaceae) is a tiny plant growing out of crevices on rocky slopes and has a narrow distribution [1]. To the best our knowledge, besides our group, no one else has carried out a study on this species. Here we report the isolation and structural elucidation of three compounds from an ethanolic extract of the dried underground part of T. orientale. The ethanolic extract was fractionated according to a previously described procedure [2]. From the ether-soluble part, compound 1 was isolated. Spectral data as well as comparison with an authentic sample has confirmed its identity as fangchinoline [2]. Fangchinoline (1) was the most abundant alkaloid in the underground parts of T. orientale. Fangchinoline was first isolated from the Chinese drug Han-fung-chi. Then, the isolation of this alkaloid from some members of the family Menispermaceae such as Stephania hernandifolia, S. tetrandra, Cyclea peltata, C. barbata, Cissampelos pareira, Triclisia subcordata, and Daphnandra species (Monimiaseae) was reported [3, 4]. This is the first report on the occurrence of fangchinoline in the genus Thalictrum. Fuzitine (2) was obtained as a dark-brown residue. This aporphine derivative is a minor component of the quaternary alkaloid fraction. Comparison of its spectral and physicochemical features with those found in the published literature established the identity of this alkaloid as fuzitine. It was previously reported to be present in Aconitum carmicheali Debx [5]. This is the first report of its occurrence in the genus Thalictrum. The third alkaloid obtained from the quaternary and ether-soluble nonphenolic alkaloid fraction was determined as berberine. Spectral data as well as comparison with an authentic sample confirmed its identity.