Two new steroidal alkaloids, (-)-16α-hydroxybuxaminone (3β-dimethylamino-16α-hydroxy-9,10-secobuxa-9(11),10(19)-dien-20-one) and (+)-cyclobuxomicreinine (3β-methylaminobuxa-5,16-dien-20-one) have been isolated from the roots of Buxus sempervirens and the leaves of B. longifolia, respectively. © 1992.