Four new glycosida (three cardenolides and a lignan), nine previously reported cardenolide glycosides and a known triterpenoid were isolated from the ethyl acetate extract of the aerial parts of Asclepias subulata. The elucidation of the structures and stereochemistry of the new glycosides has been accomplished using mainly 'H and '% NMR and mass (EI and FAB) spectral data of their acetyl derivatives and comparison of these data with those of known glycosides from the same plant as well as from other plants. The new compounds were identified as 16c+hydroxycalactin, 3/l-(@ glucopyranosyloxyt19carboxy-14B-hydroxycard-20(22)enolide, coroglaucigenin 3/?-D-glucoside and 4-(8-D-ghK.opyranosyloxy)-larciresinol.