Eight new cardenolide glycosides, 19-deoxyuscharin (1 b), 4'β-hydroxygomphoside (1 k), 3'-didehydrogomphoside (1 d), 3'-epigomphoside (1 e), 3'-epiafroside (2e), 3'-epigomphoside 3'-acetate (1 f), 3' didehydroafroside (2d), and 3'-epiafroside 3'-acetate (2f), have been isolated from Asclepias fruticosa R. Br. The structures of all new compounds were established by spectral comparisons with known compounds. The chirality at C-3' of uscharin (3b), voruscharin, and 19-deoxyuscharin (1 b) is proposed to be S. By comparison with 4'β-hydroxygomphoside (1 k), the β configuration of the 4'-hydroxy group of calotoxin (3k) (from Calotropis procera) has been established.