Three new bromotyrosine alkaloids, lipopurealins D and E, and purealidin H, have been isolated from the Okinawan marine sponge Psammaplysilla purea and the structures elucidated on the basis of 2D nmr and fabmsims data. Marine sponges belonging to the order Verongidae have proven to be a rich source of bromotyrosine alkaloids (1), among which lipopurealins A-C, isolated from the Okinawan marine sponge Psammaplysilla purea Carter (Aplysinellidae) (2) are unique metabolites with a long acyl chain. In our search for bioactive substances from Okinawan marine organisms (3-5), extracts of this sponge were further examined to obtain two new lipopurealin congeners, lipopurealins D [1] and E [2], and a new bromotyrosine derivative, purealidin H [3]. We describe herein the isolation and structure elucidation of 1-3.