<jats:title>Abstract</jats:title><jats:p>Two new biogenetically related yuzurine‐type <jats:italic>Daphniphyllum</jats:italic> alkaloids of the rare (14<jats:italic>R</jats:italic>,15<jats:italic>S</jats:italic>) series, daphmalenines A (<jats:bold>1</jats:bold>) and B (<jats:bold>2</jats:bold>), have been isolated from the leaves of <jats:italic>Daphniphyllum himalense</jats:italic>. Their structures and absolute configurations were elucidated by a combination of spectroscopic data, X‐ray crystallographic, and computational methods. A plausible biosynthetic pathway of <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> was also proposed.