Two new biogenetically related yuzurine-type Daphniphyllum alkaloids of the rare (14R,15S) series, daphmalenines A (1) and B (2), have been isolated from the leaves of Daphniphyllum himalense. Their structures and absolute configurations were elucidated by a combination of spectroscopic data, X-ray crystallographic, and computational methods. A plausible biosynthetic pathway of 1 and 2 was also proposed.