<jats:title>Abstract</jats:title><jats:p>In this article, we report on the alkaloid profile and dynamic of alkaloid content and diversity in two <jats:italic>Narcissus</jats:italic> plants at different stages of development. The alkaloid profile of the two <jats:italic>Narcissus</jats:italic> species was investigated by GC/MS and HPTLC. Fifty eight Amaryllidaceae alkaloids were detected, and 25 of them were identified in the different organs of <jats:italic>N. tazetta</jats:italic> and <jats:italic>N. papyraceus.</jats:italic> The alkaloid 3‐<jats:italic>O</jats:italic>‐methyl‐9‐<jats:italic>O</jats:italic>‐demethylmaritidine is tentatively identified here for the first time from the Amaryllidaceae family, and four alkaloids (tazettamide, sternbergine, 1‐<jats:italic>O</jats:italic>‐acetyllycorine, 2,11‐didehydro‐2‐dehydroxylycorine) are tentatively identified for the first time in the genus <jats:italic>Narcissus.</jats:italic> The different organs of the two species analyzed showed remarkable differences in their alkaloid pattern, type of biosynthesis, main alkaloid and number of alkaloids. Lycorine‐type alkaloids dominated the alkaloid, metabolism in <jats:italic>N. papyraceus</jats:italic>, while alkaloids of narciclasine‐, galanthamine‐ and homolycorine‐types were found only in the species <jats:italic>N. tazetta</jats:italic> L.