Eight new compounds including 90 -[2-amino-3-(400-O-methyl-a-rhamnopyranosyloxy) phenyl]nonanoic acid (1), 90 -[2-amino-3-(400-O-methyl-a-ribopyranosyloxy)phenyl] nonanoic acid (2), 110 -[2 amino-3-(400-O-methyl-a-rhamnopyranosyloxy)phenyl]undecanoic acid (3), 110 -[2-amino-3-(400-Omethyl-a-ribopyranosyloxy)phenyl]undecanoic acid (4), 8-(40 -O-methyl-a-rhamnopyranosyloxy)-3,4 dihydroquinolin-2(1H)-one (5), 8-(40 -O-methyl-a-ribopyranosyloxy)-3,4-dihydroquinolin-2(1H)-one (6), 8-(40 -O-methyl-a-rhamnopyranosyloxy)-2-methyquinoline (7), and 8-(40 -O-methyl-a-ribopyranosyloxy)-2-methylquinoline (8) were isolated from Actinomadura sp. BCC27169. The chemical structures of these compounds were determined based on NMR and high-resolution mass spectroscopy. The absolute configurations of these monosaccharides were revealed by the hydrolysis of compounds 7 and 8. Compounds 3 and 8 exhibited antitubercular activity at MIC 50 mg/mL. Only compound 3 showed cytotoxicity against KB cell at IC50 18.63 mg/mL, while other isolated compounds were inactive at tested maximum concentration (50 mg/mL)