Beauvericin (BEA), a toxic cyclodepsipeptide, was purified from corn kernel cultures of a toxigenic strain of Fusarium proliferatum, isolated from corn ear rot in northern Italy (45 mg/kg dry culture). The strain, designed ITEM-1503, also produced fumonisin B₁ (2,250 mg/kg dry culture), and moniliformin (150 mg/kg dry culture). Thin-layer chromatography, high-performance thin layer chromatography, high-performance liquid chromatography, low-resolution electronic impact mass spectrometry and ¹H, ¹³C nuclear magnetic resonance spectroscopy were used for BEA isolation and confirmation. This is the first report on the production of BEA by F. proliferatum.