The fruit of T. revolutum DC. yielded nineteen alkaloids which were identified by spectral and chemical methods. Nine alkaloids, argemonine, eschscholtzidine, thalicarpine, N-demethylthalphenine, thalmelatine, allocryptopine, O-methylthalicberine (1), revolutinone (2) and neothalibrine (3) were isolated from the tertiary nonphenolic fraction, while the tertiary phenolic fraction afforded platycerine, thalirevoline, thalipine and N-methylcoclaurine. From the quaternary alkaloid fraction, argemonine methochloride (8), eschscholtzidine methochloride (9), armepavine methochloride (10), berberine chloride (11), choline chloride (12) and N-methyl-6,7-dimethoxyisoquinolinium chloride (13) were identified.