<jats:p> Aspergillus tamarii Kita. isolated from seeds of Paspalum scrobiculatum L. is found to produce ergot alkaloids in submerged culture. The cultrue filtrate and mycelium are observed to contain 0.125 mg/mL and 1.2 mg/g (dry weight) total alkaloids consisting of 86.5 and 91.3% fumigaclavine A, respectively. The identification of the compound was confirmed by high-performance liquid chromatography, ultraviolet, infrared, and mass spectrophotometry analyses. This is the first report of the production of ergot alkaloid by this fungus. The possible role of fumigaclavine A as a mycotoxin is discussed.