In the previous work(1) the author succeeded in the production of a new, physiologically active ergot alkaloid, “agroclavine”, C16H18N2, m. p. 203, 5° (dec.), [α]D-153°, [α]5461-180° (C 0.98 in CHCl3), by the culture on an industrial scale. The alkaloid was obtained from the mycelium and the culture medium at the rate of 0.2-0.6% and 0.02-0.03%, respectively, when the fungus was grown at 26° for a month in a 3 L. Bernhauer flask containing 1.2 L. of the culture medium consisting of: mannitol 50 g., ammonium succinate 7 g., KH2PO4 1g., MgSO4. 7H2O 0.3 g., and water 1000 cc. (pH 5.2). It has further been found that the fungus productive of agroclavine on the culture medium produces no other alkaloid than this agroclavine both on the culture medium and on the host plants — the Agropyrum, Trisetum, Festuca, etc. grown in Japan or Manchuria, while those productive of such alkaloids as hitherto known to us produce agroclavine neither in nature nor on the culture medium. These findings have fairly enabled the author to clarify various situations under which the alkaloids are produced by an ergot fungus. The resume of the present work will appear in the next number. (1) This Journal, 22 2, 61, 85, 87 (1948). © 1949, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.