Flavonoids from Centaurea incana (Asteraceae)

Biochemical Systematics and Ecology
1997.0

Abstract

We report here from the Algerian variety of C. incana, 14 known flavonoids of which five are described for the first time in the genus Centaurea. The five new compounds are: 7,3',4',5'-tetramethyltricetin 1, 7,3',5'-trimethyltricetin 2, 3,5'-dimethyl myricetin 7-O-glucoside 3, 2"-O-glucosyl-6-C-glucosylapigenin 4, 3'-methyl myricetin 7-O-glucoside 5. The other characterized flavonoids are: 6-methoxyapigenin 6, 6-methoxyluteolin 7, 6-methoxykaempferol 8, 6 methoxyquemetin 9, apigenin-7-methylgalacturonide 10, hispidulin 7-O-glucoside 11, 6-methoxyquercetin 7-O-glucoside 12, rutin 13, 6,8-di-C-glucosylapigenin 14. The dried aerial parts of C. incana (1 kg) were successively extracted with 70% MeOH, ethylacetate and n-butanol. The concentrated extract was chromatographed on polyamide, with a gradient of MeOH in toluene. Fractions were purified by paper chromatography (Whatmann 3 MM) first with BAW (n-BuOH-HOAc-H2O, 4:1:5, upper phase) then 15% HOAc. All compounds were purified with Sephadex LH-20 prior to UV, MS and H NMR spectral analysis, colour reaction procedure, hydrolysis of the glucosides (HCl 0.1 N 2 h) and comparison with authentic samples (Mabry et al., 1970).

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