Agroclavine 1 and elymoclavine 2 are the major clavine alkaloids from Claviceps purpurea strain AA-218. Analysis of the crude fermentation product by TLC showed 5 additional minor components positive to Allport and Cocking's reagent. The two most prominent minor alkaloids (3 and 4) were purified by HPLC followed by PLC. Compound 4 was identified as setoclavine via comparison of PMR, IR, UV, and mass spectral data with published values. Compound 3 was identified as 6,7-seco-agroclavine based on IR, UV, PMR, and mass spectral data and its identity with a synthetic sample from agroclavine. The biosynthetic origin of compound 3 was discussed: while its role in clavine biosynthesis is not ruled out, it appears more likely a reductive metabolite of agroclavine, supported by the ease of chemical cleavage of the 6,7-bond in agroclavine and feeding experiments excluding its incorporation into major clavines.