Capillary zone electrophoresis (CZE) with multi-wavelength detection was used for the separation of the main betacyanins, betanin and isobetanin, and the corresponding aglycones from extracts of Beta vulgaris. Optimum separation was achieved with a fused-silica capillary tube and a citric acid-Na₂HPO₄ buffer (10 mM). The applied voltage was -22 kV and the capillary thermostating temperature was kept constant at 15°C. The results of the quantitative CZE analyses were compared with those obtained by HPLC.