Quantitative determination of cytotoxic­Friedo‐nor‐oleanane derivatives from five morphological types of Maytenus ilicifolia (celastraceae) by reverse‐phase high‐performance liquid chromatography

Abstract

<jats:title>Abstract</jats:title><jats:p>Five different morphological types of <jats:italic>Maytenus ilicifolia</jats:italic> of the same age and harvested under the same conditions showed distinct accumulations of some <jats:italic>friedo‐nor</jats:italic>‐oleananes. A rapid, sensitive and reliable reverse‐phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20α‐hydroxymaytenin, 22β‐hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range­1.0–100 µg/mL were obtained. Copyright © 2002 John Wiley &amp; Sons, Ltd.