Five new dihydroxystyrene metabolites and six known compounds of the same structural class were isolated from an association of the sponges Poecillatra wondoensis and Jaspis sp., collected from Keomun Island, Korea. The structures of novel compounds were determined to be the sodium or N, N-dimethyl guanidinium salts of a dihydroxystyrene dimer (5) and two trimers (6, 7). Two dimers (10, 11) containing imidazole moieties were also identified on the basis of the results of combined spectroscopic analyses. Several compounds exhibited weak to moderate inhibitory effects against isocitrate lyase and sortase A enzymes derived from microorganisms.