<jats:title>Abstract</jats:title><jats:p>Phytochemical investigation of the bark and leaves of <jats:italic>Pancovia laurentii</jats:italic> (Sapindaceae) resulted in the isolation of a new ceramide and a new cerebroside, named pancoviamide (<jats:bold>1</jats:bold>), and pancovioside (<jats:bold>2</jats:bold>) respectively, together with six known compounds: uracil, (<jats:italic>R</jats:italic>)‐<jats:italic>N</jats:italic>‐[(1<jats:italic>S</jats:italic>,2<jats:italic>S</jats:italic>,3<jats:italic>R</jats:italic>)‐2,3‐dihydroxy‐1‐(hydroxymethyl)heptadec‐5‐en‐1‐yl]‐2‐hydroxytetracosanamide, stigmasta‐7,22‐dien‐3‐ol, <jats:italic>β</jats:italic>‐stitosterol, <jats:italic>β</jats:italic>‐sitosterol 3‐<jats:italic>O‐β</jats:italic>‐<jats:sc>D</jats:sc>‐glucopyranoside, and 2,3‐dihydroxypropyl pentadecanoate. The structures of <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> were determined by means of spectroscopic methods. Compounds <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> were tested <jats:italic>in vitro</jats:italic> for their antiprotozoal properties against several protozoa and for their cytotoxicity.