Auramycins A and B and sulfurmycins A and B, new anthracycline antibiotics were discovered from the culture of a strain OBB-111, which was identified as Streptomyces galilaeus. The structures of the antibiotics were determined, indicating that auramycins and sulfurmycins are anthracycline glycosides of new aglycones designated auramycinone and sulfurmycinone. This report describes screening for and the production, isolation and characterization of the new antibiotics, auramycins and sulfurmycins each containing components A and B as well as the characterization of their aglycones.