In a previous paper, we reported the isolation of new anthracycline antibiotics auramycins and sulfurmycins and their aglycones auramycinone and sulfurmycinone. We now describe the isolation of 1-hydroxyauramycinone and 1-hydroxysulfurmycinone glycosides from the cultures of soil isolates Streptomyces melanogenes AC-180 and Streptomyces galilaeus AC-628. The strains were cultivated, red pigment antibiotics were extracted with a chloroform-methanol mixture, and hydrolysis of the crude extract gave aglycones identified as 1-hydroxyauramycinone and 1-hydroxysulfurmycinone via 1H-NMR, elemental analysis, and mass spectrometry. Six anthracycline glycosides were purified, including cinerubin A/B and four new ones (1-hydroxyauramycin A/B, 1-hydroxysulfurmycin A/B). The sugar moieties of the glycosides were determined by hydrolysis and comparison with authentic samples, and the antimicrobial spectrum of the new antibiotics was tested.