Nodulisporacid A (1) was isolated from a marine-derived fungus Nodulisporium sp. CRIF1, while vermelhotin (5) was obtained from an unidentified fungus CRI247-01 (a member of the Order Pleosporales). Both 1 and 5 occurred as equilibrium E/Z mixtures. Ester derivatives (2 and 3) and vermelhotin (5) showed cytotoxic activity against eleven cancer cell lines. Nodulisporacid A (1) and vermelhotin (5) exhibited moderate antiplasmodial activity.